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On the subsequent early morning, dialysate samples were gathered at twenty min intervals, and dopamine and 5-HT concentrations were quantitatively determined making use of microbore HPLC-ECD as explained elsewhere (Baumann et al. 2011). Rats had been randomly assigned to teams obtaining both drug or saline injections. The moment a few steady baseline samples have been acquired, rats acquired two sequential intravenous (i.v.) injections of drug: a person dose at time zero, followed by a threefold higher dose sixty min later on. Manage rats gained sequential i.v. injections of saline (1 mL/kg) based on the same routine. Microdialysis samples were being gathered each twenty min through the entire article-injection period of a hundred and twenty min. Ambulation within the arena, described as motion from the horizontal airplane, was quantified in 20 min bins that coincided with selection of dialysate samples. At the conclusion of the experiments, rats ended up euthanized with CO2 and decapitated. The Mind sections have been examined to validate placement of microdialysis probe guidelines throughout the nucleus accumbens. Only those rats with suitable placements had been A part of knowledge analyses.
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microdialysis experiments, neurotransmitter and behavioral info from individual rats ended up normalized to Click here to order percent Command values depending on a few preinjection samples.